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    • 专利摘要:
    The present invention relates to a device for producing a photosynthetic culture comprising at least one photobioreactor forming an enclosure provided with feed / discharge means and comprising: an aqueous liquid comprising a photosynthetic culture, at least one means for fluid supply and evacuation of said enclosure cooperating with a management system, - at least one light distributor comprising: - at least a first wall arranged to receive light at a proximal end, - at least a second arranged wall for emitting at least a portion of the light received, - a sealed cavity delimited by said at least first and second walls, - at least a portion of the emitting wall is immersed in the aqueous liquid comprising the photosynthetic culture, - at least one fluid filling at least in part said sealed cavity, - a means of recovery of said photo-bioreactor able to limit the evaporation of said aqueous liquid, characterized in that said covering means is provided with at least one opening, adapted to maintain said at least one light distributor in a fixed position in said chamber of the photo-bioreactor.
    公开号:FR3029209A1
    申请号:FR1461778
    申请日:2014-12-02
    公开日:2016-06-03
    发明作者:Frederic Barbarin
    申请人:Frederic Barbarin；
    IPC主号:
    专利说明:

    [0001] TECHNICAL FIELD OF THE INVENTION [0001] The invention relates to the field of photosynthetic culture production devices, and especially by means of a photo-bioreactor. STATE OF THE PRIOR ART [0002] The culture of microalgae is at the heart of recovery processes. Indeed, micro-algae are an alternative source especially for the production of biofuel, biomass, oils, proteins, esters or ethanol. In addition such crops can be used for the recovery and purification of wastewater, the treatment of fertilizer discharges, CO2, NO, SOUND, certain factories. The development of photosynthetic micro-algae cultures, however, requires the establishment of specific production systems, to be adapted according to the intended application, the industrial operating conditions, the strain to be cultivated, the geographical, climatic or agricultural conditions. sunshine. In addition, a well-known difficulty in this field relates to the penetration of light in the photo-bioreactor. In fact, on a given area of land, a simple horizontal water body, such as the "raceways" (photo-bioreactor large open basin, shallow), only partially uses the solar potential since, from a few centimeters depth, the opacity of the water according to its concentration of micro-algae, limits the penetration of the light in depth. The surplus of direct light that can not pass through the opacity of the culture medium (or algal water) is then partially absorbed by it in the form of heat, instead of being used for the growth of microalgae, causing a problem of evaporation of water. [0005] In addition, raceways can generate problems linked to external contaminations (bacterial, predatory, introduced competing algae, etc.) which impede production. Another disadvantage of "raceways" concerns the high rate of evaporation of water. To alleviate these problems, photo-bioreactors thinly sealed layers have been developed. However, a cooling system is essential to avoid alteration of micro-algae, making the management of the crop more complicated and reducing its yield. Indeed, these photobioreactors require complex management equipment in proportion to the volume of water exploited, which implies that their industrialization is impossible for mass production. [0007] Another embodiment has been developed in which the photobioreactors use light diffusers within them in order to increase the volume of water useful for the production of microalgae. Such devices are described in the international application W02013 / 063075, in which, LEDs have been arranged inside the photo-bioreactor to increase the yield of the photosynthetic culture. In addition, some of the bioreactors of the prior art have a relatively complex construction and sometimes use complex optics to illuminate the algal water. This type of device requires a power supply increasing the cost of microalgae culture. Therefore, sunlight is often preferred. The international application W02009 / 116853 presents an alternative light diffusers, in this case solar light by adding to the tank a transparent rigid device comprising at least one light receiving surface arranged to receive the light. solar light and at least one emission surface, immersed in the algal water, arranged to emit at least a portion of the light received in the algal water. This type of photo-bioreactor in addition to using thick-walled materials and expensive, requires significant exposure to solar radiation, which increases the evaporation of algal water and makes more complicated the management of photosynthetic culture . In addition, such light diffusers have a very large space requirement regardless of the type of tank used. This makes their maintenance, replacements and uses in industrial size photobioreactors difficult. SUMMARY OF THE INVENTION [0014] The purpose of the invention is to remedy the disadvantages of the state of the art and, in particular, to propose a device for producing a photosynthetic culture comprising at least one photo-bioreactor forming an enclosure provided with means feeding / evacuation device comprising: an aqueous liquid comprising a photosynthetic culture, at least one fluid supply and discharge means of said enclosure cooperating with a management system, at least one light distributor comprising at least one first wall arranged to receive light at a proximal end, at least one second wall arranged to emit at least a portion of the light received, o a sealed cavity delimited by said at least one first and one second wall, where at least a part of the emitting wall is immersed in the aqueous liquid comprising the photosynthetic culture, at least one fluid filling at least in part said sealed cavity, a means for covering said photo-bioreactor able to limit the evaporation of said aqueous liquid, characterized in that said covering means is provided with at least one opening, adapted to maintain said at least one minus a light distributor in a fixed position in said chamber of the photo-bioreactor. By "aqueous liquid comprising a photosynthetic culture" is preferably meant any microalgae culture; the micro-algae are chosen according to the intended application (for example the production of proteins, oil, ethanol, biomass) and culture conditions, particularly geographical, climatic and temperature conditions. In addition, the term "aqueous liquid comprising a photosynthetic culture" may be replaced by "algal water" without affecting the scope of the protection afforded by the present application. By "fluid" is meant any liquid or gas suitable for the operation of the photo-bioreactor or the development of photosynthetic culture. By "light distributor" means any body having at least two walls, the first receiving a light radiation, preferably solar, the second emitter of at least a portion of the light radiation. Preferably, said body is a cylinder or a variation of a cylinder extending for its height, along an axis X orthogonal to the support plane on which said enclosure of said photo-bioreactor. In addition, the height of said at least one light distributor is close to the height of said enclosure. That is to say, the height of said at least one light distributor is between 100% and 85% of the height of said enclosure. By "proximal end" means the end furthest from the support plane for normal use of said at least one light distributor, that is to say when it is immersed in the photo-bioreactor X axis. [0019] By "distal end" is meant the end closest to the support plane for normal use of said at least one light distributor. [0020] By "covering means" is meant all means for closing the upper surface of said photo-bioreactor to form a sealed enclosure, preferably hermetic, for example, a cover, a cover, or any other technically equivalent means. In addition, the covering means may be transparent or opaque, colored, and may be made from a cut and welded polymer strip or in one piece. Advantageously, a form of cooperation between at least one edge of said opening of said covering means and a surface relief of said at least one light distributor is carried out in order to maintain said at least one light distributor in a fixed position. in said chamber of the photobioreactor. Preferably, said light receiving wall of said at least one light distributor is disposed outside the volume defined by said chamber of the photo-bioreactor. In other words, at least a portion of said at least one light distributor is not covered by the covering means and protrudes from said covering means, so that the plane formed by said means of covering separates said at least first and second walls of said at least one light distributor. Advantageously, the number (nbestributeur), the immersed height (Hdispenser) of said at least one light distributor and the spacing (d) between the different light distributors are determined from the following formulas: In a section enclosure Circular or rectangular cross-section of S surface area (in m2), nbd. Enclosed (1) utstnuuteur 71-.D2: the volume fraction occupied by the light distributors, relative to the total volume of water in the enclosure. The fraction e is preferably between 0.6 and 0.8. D: the diameter of the light distributor (in m). This previous equation does not take into account edge effects.
    [0002] H 1, Liistri = vater = (17.qsoi, ire - q2) D 4.q2 (2) H, ist, upright: Height of the second wall of the light distributor (submerged part) (in m) q solar: flux incident of a sunny day (in! moles of photons / m2.s-1). q2: desired luminous flux at the light emitting wall of the light distributor for the good production of a given microalgae strain (in pmol / m2.s-1). / 7: the transmission efficiency between the first light receiving wall and the second transmitting wall of the light distributor.
    [0003] The height H.sub.i, ute ur can be between 1m and 12m, preferably between 4m and 8m, while the diameter of the light distributor can be between 1m and 3m. d = tc.D2 cos 30 (3) d: distance in meters between the longitudinal axes X of 2 light distributors. Preferably, said at least one light distributor is provided with fluid supply and extraction means. Said fluid supply and extraction means may be independent, partially or totally merged with each other. Preferably said at least one fluid filling at least in part said sealed cavity is composed of water and air. Thus, the immersion of said at least one light distributor into the algal water is controlled by the water / air ratio present in said cavity of said at least one light distributor. In addition, said fluid filling at least in part said sealed cavity is able to pressurize the internal volume of said at least one light distributor so as to seal the shape of cooperation between the edge of said opening and said relief surface of said at least one light distributor. Moreover, said light distributors are floating and allow to support the covering means above the algal water plane at a distance between 10cm and 50cm, thus allowing the flow of gas between the surface of the algal water and the means of recovery. Advantageously, said at least one light distributor is provided with an anchoring means disposed at a distal end. By "anchoring means" means any mass attached or not to the bottom of the photo-bioreactor, any connecting means between said at least one light distributor and the bottom of the photo-bioreactor such as a rope, a chain, or any other means known to those skilled in the art, capable of immobilizing in a desired position said at least one light distributor. According to another feature, said walls of said at least one light distributor are formed by at least one flexible membrane with preferably a high degree of transparency and resistance to elasticity. Such a flexible membrane may be made of polymer, for example polyethylene, polypropylene or polyamide, or PVC, or a multilayer of polymers having properties that complement each other. Thus, according to this feature, said at least one light distributor is inflatable / deflatable. That is to say, to obtain its form of use, said at least one light distributor must have said sealed cavity filled with fluid, in particular water and air, and have a pressure greater than atmospheric pressure. preferably about 50 hectopascals. Furthermore, the polymer of the membrane may have a degree of oxygen sealing, over its entire surface or locally, to limit the porosity of the walls of the light diffuser. Following the improvement of air management in the proximal portion of the light diffuser, several routes can be selected. Either to try to limit as much as possible the gas exchanges with the external environment in order to reduce the air intake to maintain the light diffuser at its immersion level. In this solution, the porosity with oxygen could be for example less than 0.1 cm3 / m2.day-1. On the contrary, to facilitate the evacuation of oxygen from the algal water to the water of the light diffuser. In addition, when said sealed cavity of said at least one light distributor does not contain fluid, especially when it is stored or not yet installed in said photo-bioreactor, said at least one light distributor can be folded, rolled in order to limit its space congestion. Advantageously, said polymer membrane has received or comprises during its manufacture at least one internal and / or external surface treatment among the following: anti-drip, anti-dust, UV filter and / or infrared, UV stabilizers, diffusing, semi-reflective, hydrophobic, anti-dust, anti-algae effect (for example: titanium oxides). For example, a treatment with titanium oxides makes it possible to avoid, on the first wall, receiving solar light, the formation of condensation drops capable of disturbing the passage of light in said at least one light distributor and on the second wall to avoid the phenomenon of "fouling", that is to say the deposition of algae on the walls. Treatment on the inner surface with a long infrared filter keeps the long infrared in the light distributor to heat the interior by greenhouse effect. An anti-UV treatment prevents the destruction of the photosynthetic culture by UV. A semi-reflective treatment allows better routing of the light to the distal portion of the light distributor by a "mirror effect", the intrinsic light absorption should not exceed 10%. This local treatment is located on the proximal side on the second wall inside the sealed cavity of the light distributor, and can be achieved by dipping or spraying a metallic material. According to another characteristic, said photo-bioreactor comprises at least one bubbling means with CO2 input, arranged at least on part of the bottom of the enclosure of the photo-bioreactor, so as to allow a permanent mixing of said aqueous liquid comprising said photosynthetic culture. By "bubbling means" is meant any means known to those skilled in the art capable of providing within said chamber gas, especially in the form of bubbles. BRIEF DESCRIPTION OF THE FIGURES [0042] Other features, details and advantages of the invention will emerge on reading the description which follows, with reference to the appended figures, which illustrate: FIG. 1 reveals a 3D representation of a Embodiment according to the invention, FIG. 2 discloses a 3D representation of an embodiment of the photobioreactor without the covering means, FIG. 3 is a detailed view of an embodiment of a dispenser of light installed in a photo-bioreactor according to the invention, maintained in a fixed position by the covering means, FIG. 4 is a longitudinal section of an embodiment of a light distributor, FIG. FIG. 6 is a diagram of the proximal end of one embodiment of said light distributor, FIG. 7 is a schematic orthogonal section. in the plane formed by the support of an embodiment of the photo-bioreactor fluid supply and extraction / discharge network, FIG. 8 discloses a three-dimensional representation of a large-scale embodiment of a production device according to the invention comprising several photo-bioreactors. For clarity, identical or similar elements are identified by identical reference signs throughout the figures. DETAILED DESCRIPTION OF AN EMBODIMENT [0044] FIG. 1 illustrates an embodiment of the invention in which the device for producing a photosynthetic culture 1 comprises at least one photo-bioreactor 2 forming an enclosure 3 provided with feed / discharge means 4, and able to contain an algal water. For example, the enclosure may be a natural cavity such as a lake, a pond, or in the sea or artificial, concrete, sheet, geo-membrane, etc.. It can be painted white thus allowing the reflection of light, coated with a waterproof material, and / or thermally insulated from the inside and / or the outside. Said photo-bioreactor may be cubic, parallelepipedic, cylindrical, spherical, pyramidal, prismatic or all variants thereof. Preferably, one will choose a parallelepipedal or cylindrical shape whose dimensions are adapted to the industrial production of a photosynthetic culture. As an example for a parallelepipedic photo-bioreactor: 100m over 30m and 8m height; or for a small cylindrical photo-bioreactor: 7m diameter and 4m height. Of course, the dimensions of the photo-bioreactor will have to be determined and adapted according to the type of industrial installation desired. Preferably, said photo-bioreactor is above ground; or in order to limit the space congestion of such an installation, it can be partially buried. It can be placed near a CO2 emitting plant, a wastewater treatment plant, methanisers / digesters, in the context of valuations (biogas, cogeneration, composting, CO2 recycling ...). In addition, it can be placed on arid lands or deserts, near the sea for water supply. Said enclosure 3 is covered at its proximal end by a covering means 5, sufficiently resistant to at least tolerate the weight of a man once installed. For example a plastic tarpaulin or a combination of polymer layers, or even a polyester-type waterproof fabric with a PVC coating typically used for roofing tents or trailers trucks. The thickness of the covering means 5 is for example 0.6mm with a weight of about 600g / m2. In addition, the covering means 5 has at its periphery a fastening means 21 to allow sealing application on the walls of the enclosure 3. This may be for example a hem with eyelets in which passers, or many sleeves in which metal tubes are inserted. In addition, it may be advantageous for its density to be less than 1 so that it can float in order to facilitate its installation. Said photo-bioreactor 2 is provided with at least one light distributor 6. Said at least one light distributor 6 is preferably tubular, cylindrical, extending longitudinally along an axis X, of which the dimensions are adapted to the dimensions of the photo-bioreactor. The tubular shape of the light distributor 6 was chosen because it is simple and easily achievable in the factory, even for large dimensions, compared to other geometric shapes. Said at least one light distributor or "balloon" 6 is delimited by a flexible membrane preferably made of polymer, capable of varying the volume of said at least one light distributor 6 such as an inflatable / deflatable balloon and has at least two walls, at least one first wall 7 arranged to receive light at a proximal end, at least one second wall 8 arranged to emit at least a portion of the received light. The flexible membrane is preferably slightly extensible and inelastic, to minimize the phenomenon of bloating on the tubular portion of the balloon 6. The thickness of the membrane may be for example about 200pm. The membrane for producing the sealed cavity of the light distributor 6 may consist of several parts welded together. For example, a long tubular part with two welded ends. The many transparent films used in particular for greenhouses, are very economical because manufactured in large quantities by inflation extrusion method or co-extrusion. These manufacturing processes are very advantageous because not only are the production costs lowest, but they make it possible to form multilayer films combining various characteristics. Those skilled in the art will know how to use these known processes to manufacture the transparent film adapted to the constitution of the walls 7 and 8 of the light distributor 6. In addition, the degree of overall light transmission of the membrane 7, 8 is preferably greater than 90%. The diffusing effect of the polymer must alter as little as possible its degree of light transmission. Light transmission is an important property of the membrane. Nevertheless, it is necessary to distinguish the difference between the direct light and the indirect light also called diffused. Too much direct light could result in destruction of microalgae, and absorption losses in caloric form, especially in areas with high solar radiation. The incorporation of mineral fillers into the membrane makes it possible to obtain a better distribution of the light which becomes more diffuse and more homogeneous. In addition, co-polymers (EVA type) have the function of blocking long infrared radiation, and slow down the temperature drops. The light receiving wall 7 at the proximal portion of the light distributor is preferably of slightly convex shape. This shape allows a better capture of light relative to the position of the sun all day long. The dimensions, the number of light distributors 6 and the distance between each light distributor 6 are determined from the following formulas: 4.e.sen '. ,,. ,, "distributor = 7 r .D2me YI D i" distributor = '174 solar - q2) (2) 4.q2 d = II 71-.D2 (3) 44. cos 30 With: H distributor: Height of the second wall 8 of the light distributor 6 (immersed part) (in m) D: the diameter of the light distributor 6 (in m). q solar: incident flux of a sunny day (in! moles of photons / m2.s-1). q2: desired luminous flux at the light emitting wall of the light distributor for the good production of a given microalgae strain (in pmol / m2.s-1). ii: the transmission efficiency between the first light-receiving wall 7 and the second emitting wall 8 of the light distributor. ndistributor: the number of light distributor 6 in enclosure 3 of photobioreactor S enclosure: surface of an enclosure of circular or rectangular cross-section (in m2) e: the volume fraction occupied by the light distributors, relative to the total volume of water in the enclosure. The fraction e is preferably between 0.6 and 0.8. d: distance in meters between the longitudinal axes X of 2 light distributors.
    [0004] By way of illustration, for an epsilon fraction e equal to 0.6857, and when the diameter D of each distributor is equal to 2 meters, the distance d between the longitudinal axes X of 2 directly adjacent light distributors is 2.30m, ie 30cm d space between the walls as shown in Figure 5.
    [0005] The distance d can be between, for example, 1m and 3m. The epsilon e value is also partly proximal, the area covered by all of said light distributors relative to the total area of the photo-bioreactor 2. A e = 0.667 for example, 2/3 of the surface of the enclosure 3 is covered by the first wall 7 of the light distributor 6, the last third being covered by the covering means 5. For a fraction e between 0.6 and 0.8, the water contained in the light distributors represents therefore approximately 60% to 80% of the total volume of water in the enclosure 3, and the algal water will represent 20% to 40% of the total water volume. Of course, the dimensions mentioned in the present application of the photo-bioreactor 2 and the at least one light distributor 6 are not exhaustive and may change depending on the performance obtained and ergonomics sought. The number of light distributors may for example be between 1 and 10,000 per chamber 3. The light distributors 6 are arranged equidistantly from each other in the chamber 3 as shown in FIG. The arrangement forms a succession of staggered rows, i.e., one row is shifted by half the distance between two light distributors with respect to the preceding row and the next row. This arrangement optimizes the useful luminous surface in the algal water volume. The advantage of introducing light distributors in algal water is primarily to manage the large amount of light flow in excess of the sun. In a "raceway" type of pond, on a sunny day, the amount of light arrives directly on a horizontal body of water. If a portion of the photon quantity is absorbed by photosynthesis, much of the light energy will be lost as heat. Indeed, micro-algae up to a certain threshold do not absorb too much brightness, and conversely it can generate photo-inhibition. In addition, an excess of surface water temperature can inhibit the growth of microalgae and much water is lost through evaporation. A three-dimensional approach makes it possible to circumvent these problems by diffusing the luminous flux over a maximum area so as to increase the quantity of photons captured by the micro-algae. Also, the penetration of light to the distal portion of the balloon 6 is decisive for the good photosynthetic production, hence the interest of a membrane with appropriate characteristics to capture the best sunlight flux, and distribute it as evenly as possible over the entire surface of the second wall 8 emitting light. The amount of solar light varies according to the geographical latitude of the location of the production site. The illumination of a sunny summer day in France is 80 000 Lux, a solar incident flux of about 1350 pmol / m2.s-1. Microalgae require only a few moles of photons / m2.s-1 or a few tens of pmoles / m2.s-1 to overcome the compensation point between respiration and photosynthesis, and thus start to grow and to duplicate. The table below shows the results of various published studies on the daily production of various micro-algae, according to the intensity of the luminous flux: Microalgae strain Concentration Biomass volume production (g / L / day) Production Luminous flux (umol / m2 / s) References Cellular literature in lipid (g / L / d) phase, stationary (x10E6 cell / ml) Chlorella Vulgaris 6 0.037 40% 76 Illman et al. (2000) Chlorella Vulgaris 0.485 Dragone et al. (2011) Chlorella Vulgaris 0.254 Liang et al. (2009) Chlorella Vulgaris 1,437,400 Shih-Hsin Ho et al. (2012) Chlorella Vulgaris 0.39 90 Shih-Hsin Ho et al. (2012) Chlorella Vulgaris 0.272 0.144 Yeh and Chang (2012) Chlorella Vulgaris 33.2 100 Jean Hee Bae et al. (2011) Chlorella Vulgaris 64.9 100 Jean Hee Bae et al. (2011) Chlorella Vulgaris 227.2 Zaleha & al. (2013) Nannochloropsis oculata 112.3 100 Jean Hee Bae et al. (2011) Nannochloropsis oculata 0.382 0.17 Su et al. (2011) Nannochloropsis oculata 0.161 Chiu et al. (2009) Nannochloropsis oculata (7.9g / L) 1.5 Ramanathan & al. (2011) Nannochloropsis sp. 22.5 34 Roncallo & Al. (2012) Nannochloropsis sp. 37.5 34 Roncallo & Al. (2012) Nannochloropsis sp. 0.124 0.074 Jiang et al. (2011) Nannochloropsis sp. 0.148 Cheirsilp and Torpee (2012) Nannochloropsis sp. 107.3 100 Jean Hee Bae et al. (2011) Nannochloropsis sp. 92.2 17 8h / 24 Zaleha & al. (2013) Desmodesmus sp. 0.762 0.263 700 Shih-Hsin Ho et al. (2014) Desmodesmus sp. 0.433 0.213 700 Shih-Hsin Ho et al. (2014) Desmodesmus sp. 0.67 0.302 700 Shih-Hsin Ho et al. (2014) Scenedesmus obliquus 0.151 60 Shih-Hsin Ho et al. (2010) Scenedesmus obliquus 0.201 60 Shih-Hsin Ho et al. (2010) Nannochlorum sp. 250 80 Pereira et al. (2011) Tetraselmis sp. 40 80 Pereira et al. (2011) Tetraselmis sp. (12.38g / L) 0.173 30 1211/24 Mohd Shamzi & al. (2013) Chaetoceros calcitrans Robert & His (1987) Chaetoceros calcitrans 14.7 35.40% Kwangdinata et al. (2014) Chaetoceros calcitrans (8.1 g / L) 1.7 Ramanathan & al. (2011) Chaetoceros calcitrans Kaspar et al. (2013) Chlamydomonas sp 7.5 (4g / L) 0.169 250 Nakanishi & al. (2013) Spirulina Platensis (1.59g / L) 52 Chauhan & Pathak (2010) Spirulina Platensis (1.84g / L) 77 1211/24 Gami & al. (2011) [0062] Although studies are very often carried out on small volumes in the laboratory, the disparity of production performance for a given illumination 5 is great. Some studies, however, show good volume yields with a low illumination of a few tens of moles of photons / m2.s-1 only. One of the studies by Roncallo & al. (2012) gives a maximum concentration of microalgae Nannochloropsis sp. of 37.5x106 cells / mi for a brightness of about 34 pmol / m2.s-1 (2000 Lux) only, in a photo-bioreactor with a vertical column of 25 cm in diameter. One of the studies by Ramanathan & al. (2011) shows a daily production of Chaetoceros Calcitrans up to 1.7 g / L.day-1, for a luminosity of only 21 pmol / m2.s-1 (1200 Lux), in vertical tubes of 4.5 cm in diameter. In the current literature, the yields in surface production in g / m2.day-1 are disparate. The results range from a few grams per m2.day-1 to 130 grams per m2.day-1. The Indian study of Sudhakar & al. (2012) shows, for example, surface yields in conventionally-run conventional tanks averaging 73 g / m2.day-1 and 76 g / m2.day-1 on two separate biomass production sites. By taking again equation (2) with the values D and q 'lai' sus mentioned as examples, starting with a quantity of light q2 emitted on the surface of the second wall 8 for example 85 pmol / m2.s sufficient for the proper growth of a given micro-alga strain, and a transmission efficiency ri 80% in the sealed cavity of the balloon 6, the height H of the second wall 8 of the light distributor will then be worth 6m. Luminous flux q2 emitted in the distributor with D = 2m H-6m -80% cloud Overcast Good weather Illuminance: 25000 80000 Lux Flux incident solar q: 430 1377 pmol / m2 / s q2 distributor: 26 85 pmol / m2 / s [ 0065] The absorption of the visible radiation in the water column height of the light distributor 6 should be the lowest in order to keep a good efficiency ii transmission of light. That is, to have filtered water with the lowest turbidity. In water, the red light in the wavelength range of 600 to 700 μm is absorbed more rapidly than the blue light (400 to 500 μm). At 10m depth, about 80% of the blue light is still available in clear water, while the red will be fully absorbed.20 However, all microalgae photo-synthesize among other blue light. Those skilled in the art will obviously take into account, depending on the desired water level in the chamber 3. For example, in diatoms, the production of lipids is favored by the blue wavelengths, precisely those which are the least rapidly absorbed by water. The water contained in the sealed cavity of the balloon 6 may be freshwater or seawater. Most of the substances composing sea salt have little effect on the absorption of light in the water. water. There is practically no difference between the absorption spectrum of a very clear seawater and that of distilled water. A maximum amount of photons of 300 pmol / m2.s-1 may be distributed on the surface of the second wall 8 of the balloon 6. For a quantity of PAR (Photosynthetically Active Radiation, expressed in pmol / m2.s- 1 or W / m2) given at a geographical latitude, the average amount of photons available in the chamber 3 obviously depends on the diameter of the light distributor in proportion to its depth, this amount is calculated according to the formula (2). In addition, the maximum concentration of microalgae in the algal water is to be adapted for optimum daily photosynthetic production. The semi-continuous mode of production is interesting. It consists of concentrating the cell density up to the stationary phase, ie the threshold at which the microalgae can no longer develop further in a culture medium and at a given brightness. At this saturation threshold, a portion of the algal water is taken by the evacuation means 4 to be filtered and to collect the biomass. The study of Shih-Hsin Ho & al. (2014) shows that a 90% removal of the algal water volume every 5 days allows a better daily volume production of microalgae Desmodesmus sp. a 10% sample every two days (0.67 g / L / day vs. 0.20 g / L / day respectively). The frequency of harvest will depend on the rate of growth of the given microalgae strain, to reach the saturation threshold, and the amount of algal water taken at each harvest. A production of biofuel can be calculated based on a theoretical volume productivity, quantified in the following table: Number of days of production: 300 days / year Diameter D of the distributors 6: 2 m Height H of the distributors 6 Volume: 6 m Emitter surface area of the distributors: 40.86 m2 Epsilon fraction value: 0.7495 Distance between distributors (with X axes): 2.2 m Number of distributors / ha (equation 2): 2386 Number of m2 per hectare : 97490 m2 / ha Algal water volume: 16539 m3 / ha Raceway depth equivalence: 17 cm Surface production in the distributor: 60 g / m2 / d Biomass biomass production: 0.354 g / L / d% of unoccupied land (roads, etc.): 15% per ha Biomass production per hectare: 1492 T / ha / year% of lipids in biomass: 30% Annual production Bio-fuel per hectare: 508000 L / ha / year [0069] In a alternative embodiment, the diameter of the first wall 7 may be different from the diameter of the second In another alternative embodiment of the invention, the first wall 7 receiving the light is made of rigid plastic of polycarbonate type, and the second wall 8 of flexible membrane type. The interface between the two walls 7, 8 is sealed. In addition, both walls can be separated for storage and / or maintenance. Furthermore, according to another embodiment, said balloon 6 is provided at its proximal end with a lifting ring or any other technically equivalent means capable of extracting said balloon (preferably in its deflated form) from said balloon. enclosure 3. [0072] In addition, said balloon 6 is provided at its proximal end with independent means 11 for feeding and discharging, able to fill and extract one or more fluids from the sealed cavity delimited by the walls 7 and 8. as shown in Figure 3. These means may be inserts welded to the wall 7 of the balloon 6. These inserts include an internal thread to allow connection to the circuits, and may be of different sizes depending on the type of fluid to be made. circulate. In a preferred embodiment, said fluids introduced into the sealed cavity are water and air, able to control the immersion of said at least one light distributor 6 in algal water. The water contained in the sealed cavity is as clear as possible and filtered, for good light transmission at the surface as well as at depth. In another embodiment, said at least one light distributor 6 has two sealed cavities separated by a transparent membrane, one for the air partly proximal, one for the water for immersion in the distal portion. In addition, so as to keep said at least one light distributor 6 immersed in a plane parallel to the axis X, in the manner of a "fishing cap", said at least one light distributor 6 is provided on its distal end with a mass 10. Said mass 10 may be metal, concrete, or a molded plastic capsule filled with sand. The shape of the mass is preferably flat, so as to take a minimum of space along the orthogonal axis X. According to alternative embodiments, said mass may be screwed into an insert, hang along a link located at the distal end of said at least one balloon 6, or preferably be at the end of a guide 9 passing right through said balloon 6, or any other technically equivalent means. In a preferred embodiment, said independent feeding and discharging means 11 consist of a means for supplying water 11a, means for discharging (or discharging) water 11b, d an air supply means 11c and an air exhaust means 11d, these means 11 may consist of valves, valves or any other means known to those skilled in the art. Figures 6 and 7 illustrate this arrangement. Thus, such an arrangement can make it possible to isolate from the supply and discharge circuit a specific distributor 6 during a maintenance phase without compromising the other light distributors 6 of said production device 1. [0078] In addition, as illustrated in FIGS. Figures 2 and 7, said water supply means 11a is provided with a so-called long pipe 13 which extends substantially along the longitudinal axis X and opens into the bottom of said balloon; said discharge means (or discharge) in water 11b is provided with a so-called short pipe 14 which opens below the plane formed by the interface between the water and the air, for example at 10 or 20 cm of said plan ; said air exhaust means 1 1 d is provided with an air discharge pipe 15 which opens on the plane forming the interface between and water and air. This air evacuation pipe 15 is able to establish the depth of immersion of the balloon 6 in the algal water by controlling the volume of air in the balloon 6. Preferably, said pipes runs 14, along 13 and said exhaust pipe 15 are attached to said guide 12; in addition, said air supply means 11c, and said air exhaust means 11d can be merged as shown in FIGS. 2, 4 and 6. [0079] The supply and evacuation means in FIG. air 11c and 11d are simple physical means for absorbing the expansion or contraction of air within the sealed cavity at the proximal portion of the light distributor 6, irrespective of the fluctuation of the daily meteorological temperature. Thus the volume of air at the proximal portion remains constant, ensuring the proper immersion of said balloon 6 in the algal water. Furthermore, the long supply pipe 13 can also be used to drain the balloon 6. In this case, it will be pierced preferably regularly over its entire length to better drain said balloon 6 and thus avoid clogging too quickly between the walls of said flask and the pipe 13. Another solution is that said pipe 13 is provided with a strainer at its end. Optionally, the air discharge pipe may have different lengths in order to obtain a height difference between for example two rows of balloons 6 in the photo-bioreactor 2. This may be particularly advantageous for creating a slight slope of the covering means 5, and thus facilitate the evacuation of rainwater. Thus the covering means may for example be further from the distal portion in the middle of the photo-bioreactor than the sides. The height difference may be slight, preferably less than 2%. Optionally, a level for measuring the height of the water contained in the sealed cavity of the light distributor 6 is connected to an external electronic control unit (not shown). This level can be a probe fixed or inserted on the guide 12 or on the long pipe 13. The connections of said supply and discharge means 11 on the evacuation means 4 cooperate with the management means via a ramp connecting several light distributors 6, for example four balloons. Then at the edge of the enclosure, the ramp is connected to the management means joining for example a room for pumping and filtration of water and air balloons. For a large enclosure, each row of balloons is for example fed by several ramps connected end to end via flexible hoses between them. Also to facilitate maintenance in a localized manner, valves are placed at the ends of the rows of balloons, and accessible from the edge of the enclosure, to isolate the row in question from the rest of the circuit. Optionally, as illustrated in FIG. 4, said at least one light distributor 6 may be provided with at least one additional holding means 20 for stiffening its walls (keeping the tubular appearance of the light distributor 6) and avoid a domed effect on the second wall. These means 19 may be straps spaced on the height of the light distributor 6 or a localized thickness of the second wall. As illustrated in FIG. 7, said photo-bioreactor comprises at least one bubbling means 9 of a gas, preferably air, in particular with additional CO2 input (with possibly a nutrient supply), arranged at least on part of the bottom of the enclosure 3 of the photo-bioreactor 2, so as to allow a permanent stirring of said aqueous liquid comprising said photosynthetic culture and to evacuate the oxygen produced by the photosynthesis out of the algal water, thus avoiding the risk of a strong oxygen supersaturation and consequently a growth inhibition of micro-algae. To do this, the speed of rise of the bubbles may preferably be between 0.3 to 0.35m / s. In addition, preferably, said at least one bubbling means 9 is not placed in line with said at least one light distributor 6; it is placed for example between two light distributor 6 [0087] Furthermore, the increase in the number of bubbling means 9 m2 has the advantage of avoiding spiral-flow phenomena. This phenomenon of ascending current of the algal water created by the rise of the bubbles which accelerates the speed of passage of these, and affects the gas exchange. The rapprochement of the bubbling means 9 between them breaks the risk of spiral-flows. The air diffused in the algal water may contain a higher concentration of CO2 than that of atmospheric air (400ppm), in order to reduce the need for aeration and its cost. For example a proportion of the CO2 in the air brought to 1000ppm would be sufficient to divide by two approximately, the needs and costs in aeration, without affecting the good evacuation of oxygen. The proximity of the production device 1 with a CO2 emitting plant, or the contribution of CO2 from biomass digesters integrated into the production system would be a wise choice to optimize production costs. The bubbling means 9 may comprise EPDM membrane diffusers for aeration in fine bubbles. It is in the form of disks attached to the air supply network. The standard air diffusers used for the aeration of the basins of the purification plants may be suitable as long as they are adapted to the water column pressure of said photo-bioreactor. In addition, in an alternative embodiment, a mechanical stirring means may be used in addition to said bubbling means 9, for example with propellers. Optionally, one or more LED lamps may be placed inside the sealed cavity of the light distributor 6, for example along the guide 12, and electrically connected. Such LED lamps can increase the daily photosynthetic production of micro-algae. The LED lamps are preferably immersed in the water of the balloon 6 for better light diffusion. They may have a power of several watts and preferentially emit in the absorption wavelength range of chlorophyll during photosynthesis (400 nm - 700 nm). This lighting can complement natural lighting on days of low sunlight, and / or operate at night, knowing that it can be interrupted according to sequences related to the biological cycles of micro-algae. As illustrated in FIGS. 3 and 5, the covering means 5 of said proximal end of the enclosure 3 of the photo-bioreactor 2 is provided with at least one opening 16 adapted to receive therein and to maintain said at least one light distributor 6 in a fixed position in said photobioreactor chamber 2. Thus, when necessary, a light distributor 6 can be replaced without having to touch said cover means or other light distributors 6. [ 0093] As shown in Figure 6 showing an alternative embodiment, the holding of said at least one light distributor 6 in said at least one opening 16 is by cooperation of mechanical shapes between a surface relief 17 of said at least one distributor 6 and an edge 18 of said aperture 16. [0094] Said surface relief 17 may be a negative relief in the wall of said at least one light distributor 6 such as a ra inure or groove in which is housed said edge 18, or a series of positive reliefs such as a rail or a flange framing said edge 18, as shown in Figure 6. In an alternative embodiment, said relief of surface 17 may be an intermediate sleeve welded between the walls 7 and 8. One or more flexible strips or semi-rigid profiles may be inserted into the sleeve to form the bead or a prestressed ring ready to be housed in said opening 16. [ 0096] In addition, the surface relief 17 may be an inflatable sheath independently of the sealed cavity of the light distributor 6. [0097] Interestingly, said fluids within said at least one balloon 6 create an overpressure, deforming the walls said at least one light distributor. This makes it possible to create a tight cooperation between the wall of said at least one light distributor 6 and the edge 18 of the opening 16 of the covering means or cover 5. In another embodiment of alternative embodiment, said at least one light distributor 6 has a diameter slightly greater than that of said at least one opening 16; and when said at least one light distributor is inflated it is trapped by the opening 16 during said shape cooperation. Under the sheet 5, the evacuation of air saturated with water (or possibly ethanol) and oxygen, is by means of evacuation 4 such that nozzles placed along said enclosure 3 without excessive pressure, to prevent leaks. These gases are eventually sent to a room to collect water, calories and oxygen. Thus, the evaporated water can flow through a condenser to drastically reduce the water supply during operation of the photo-bioreactor. Suitable equipment can condense more than 90% of the evaporated water, and thus recycle it into the algal water of the enclosure 3. This arranging process can also be associated with an air / air or air / heat exchanger. water, or a heat pump because the air leaving the enclosure 3, hotter and saturated, can easily heat the water feeding the balloons 6, or the air loaded with CO2 diffused in the algal water. According to an alternative embodiment, the evacuation means 4 can be connected directly to the cover 5. [00102] According to the invention, supply / discharge means 4 such as discharge mouths in algal water filtered are located on the walls of the chamber 3 of the photo-bioreactor, in particular towards the proximal end under the algal water plane to allow a turbulence-free filling of the chamber with respect to said at least one distributor of light 6. In addition, the discharge of algal water can be done by an evacuation network squaring the bottom of said enclosure 3, for example, under each light distributor 6. The algal water can also join a pool of water. storage or processing plant and filtration, centrifugation and biomass transformation, via appropriate collectors. All the feed and return manifolds for the algal water, the water and the air of the light distributors, and the air used for the bubbling, can go along several photo-bioreactors, be fixed in height the along the outer walls of the enclosure 3, be buried, be inserted between two walls separating two photo-bioreactors, or be part of a structured assembly separating two photo-bioreactors. In addition, the chamber of the photo-bioreactor is equipped with probes (level, temperature, composition, pH, salinity, turbidity, O2, CO2, etc.), meters and automatic control integrated in a system. Management. In a particular embodiment of the invention, the nutrients used for the development of the culture medium are injected into the algal water after filtration, that is to say on a circuit returning to the enclosure 3. [ In order to obtain an industrial-sized installation, said production device 1 may comprise a plurality of photo-bioreactors 2 whose enclosures 3 are installed side by side as shown in FIG. supply, extraction, discharge, evacuation as well as control and management means and storage means, can be shared between the different photo-bioreactors or groups of photobioreactors. [00107] Interestingly, the covering means 5 and the wall 7 of the light distributors 6 allow to obtain a greenhouse between them and the algal water plane. The principle of the greenhouse is a simple way to capture excess solar energy not used by photosynthesis of microalgae.
    [0006] Recovered as heat, it contributes to the general rise in the temperature of the culture medium. [00108] The opportunity to use a photo-bioreactor several meters deep with a large volume of water has several advantages. First, the stability of the temperature of the culture medium. Micro-algae are very sensitive to temperature variations. The James & Boriah (2010) US study shows that at a depth of 60cm, biomass production is twice as large as at 10cm deep, even with lower brightness, because of the better stability of the biomass. temperature. The inertia generated by the volume of water is therefore very important to counter daily atmospheric temperature oscillations. In addition, the volume of water in the chamber 3 can dissipate the heat captured by the greenhouse effect in the proximal part, thus avoiding excessive temperatures due to strong sunlight on the surface. The height of the water column serves as a sort of calorie accumulator. Thus, the light energy supplied to m2 by the sun is exploited more by the photobioreactor, improving its yield by prolonging the harvest season. Indeed this accumulation of heat is not lost and is used for the development of micro-algae on bad weather days. The analogy can be made to that of a greenhouse vegetable crop and a field crop: greenhouse cultivation allows almost permanent production whereas it is only a few months in the field. Another possible analogy is that the water temperature of a Plexiglas underwater pool remains higher and for longer in the season compared to an uncovered pool. Also the depth of the chamber 3 will be adapted according to the geographical location of the photo-bioreactor, the amount of solar energy varies according to the terrestrial latitude. For example, a shallow basin at the equator will have an algal water temperature that is too high for photosynthetic growth because the heat dissipation will be too low. On the other hand, the covering means 5 and the wall 7 of the light distributors 6 have an insulating function to the weather, by the air layer separating them from the body of water. Thus the temperature of the culture medium in the proximal part will be less influenced by daily weather conditions (cold winds, hot winds, heavy rains, etc.). The mixing of the algal water by aeration of fine bubbles allows the homogenization of the temperature over the entire height of the water column. Also, the circulation of the water inside the sealed cavity of the light distributors 6 is important for homogenizing the temperature from top to bottom in the enclosure 3. The slow and continuous circulation of the surface water heated by the sun towards the distal portion of the light distributor is effected by the independent means 11 for feeding and discharging. In addition, the water temperature of the sealed cavity may be regulated by a supply or a withdrawal in external calories, for example a heat exchanger or any other heating or cooling means. Thus, as an inverted "bain-marie", the regulated temperature of each light distributor is dissipated and in turn regulates the temperature of the algal water in the chamber 3. Implementation of a device according to the invention [00114] An implementation method is to slide the cover 5 over the chamber 3 full of water, by inserting the balloons 6 as and from the edge of the enclosure 3. The sheet 5 is stretched and held on the surface of the water. Balloons 6 are placed in a first row of openings 16 of the sheet 5, and are connected to each other. Air is injected to allow their floating in the water of the enclosure 3. So on for the other rows, while advancing the cover 5 to finally cover the enclosure 3. The connection between the cover 5 and the enclosure 3 is sealed. Water is added to the flasks 6 until the pressure is reached. Although the seal is already existing from that moment between said balloon 6 and the opening 16, the edge 18 of the latter is not fitted into the surface relief 17 of said balloon 6.
    [0007] For this, air is injected under the sheet 5, while the air / water ratio in the balloons 6 decreases. The pressure under the sheet 5 causes the edge 18 of the opening 16 to rise along the wall 7 of said balloon 6 until it engages in the relief 17. According to another method of implementation, the sheet 5 can be installed above the enclosure 3 previously filled with water, using a crane or mobile gantry. Said balloons 6 are placed through the openings 16 by means of a crane or a motorized gangway specially developed for this task. Said balloons 6 are deployed, connected, filled with water and air with a low air / water ratio. The membrane of said balloon 6 is then stretched by pressurization. The air / water ratio in said balloons 6 is increased so that they raise the sheet 5. The assembly makes the sheet 5 is now suspended by said balloons 6. The interlocking between the edge 18 of said opening 16 and the surface relief 17 of said balloon 6 can be effected by a force exerted downwardly on the sheet 6 by means of a tool adapted for this purpose. The floatation height of each flask 6 can be regulated by blowing air until it is discharged through a pipe 15 provided for this purpose. The level of the algal water and that of the balloons 6 can be adjusted so as to obtain a height of sheet 5 slightly greater than the edge of the wall of the enclosure 3, to facilitate the flow of rainwater towards the periphery of the photo -bioréacteur. Many combinations of the embodiments can be envisaged without departing from the scope of the invention; the skilled person will choose one or the other depending on the economic, ergonomic, dimensional or other constraints that must be respected.
    权利要求:
    Claims (10)
    [0001]
    REVENDICATIONS1. Device for producing (1) a photosynthetic culture comprising at least one photobioreactor (2) forming an enclosure (3) provided with feed / discharge means (4) and comprising: an aqueous liquid comprising a photosynthetic culture, at least one means (4) for supplying and discharging fluids from said enclosure cooperating with a management system; at least one light distributor (6) comprising: at least one first wall (7) arranged for receiving light at a proximal end, - at least a second wall (8) arranged to emit at least a portion of the received light, - a sealed cavity delimited by said at least a first (7) and a second wall (8). at least a part of the emitting wall (8) is immersed in the aqueous liquid comprising the photosynthetic culture, at least one fluid filling at least in part said sealed cavity, a means of covering said photoincrete. bioréacte ur (2) adapted to limit the evaporation of said aqueous liquid, characterized in that said covering means (5) is provided with at least one opening (16), able to maintain said at least one light distributor (6) in a fixed position in said chamber (3) of the photo-bioreactor (2).
    [0002]
    2. A photosynthetic culture production device according to claim 1, characterized in that a shape cooperation between at least one edge (18) of said opening of said covering means (5) and a surface relief (17) said at least one light distributor (6) is made in order to maintain said at least one light distributor (6) in a fixed position in said chamber (3) of the photo-bioreactor (2).
    [0003]
    3. Apparatus for producing a photosynthetic culture according to one of claims 1 or 2, characterized in that said wall (7) for receiving light from said at least one light distributor is disposed outside the volume defined by said enclosure (3) of the photo-bioreactor (2).
    [0004]
    4. Device for producing a photosynthetic culture according to one of claims 1 to 3, characterized in that the number (ndistributor) and the immersed height (Hdistributeur) of said at least one light distributor (6) and the spacing (d) between the different light distributors (6) are determined from the following formulas: ## EQU1 ## (1) e: the volume fraction occupied by the light distributors (6), relative to the total volume of water in the enclosure (3). D: the diameter of the light distributor (6) (in m). S enclosing the surface of an enclosure (3) of circular or rectangular cross section (in m2). (q-qso / area q2 (2) H distributor Height of the second wall (8) of the light distributor (immersed part) (in m) q solar: incident flux of a sunny day (in! moles of photons / m2.s-1) q2: desired luminous flux at the light emitting wall of the light distributor for the good production of a given microalga strain (in pmol / m2.s-1). transmission between the first light-receiving wall (7) and the second light emitter-distributing wall (8) z.D2 cos 30 H n, Listrictor 4.q2 D d = (3) d: distance between the longitudinal axes X of 2 light distributors (6) (in m).
    [0005]
    5. Apparatus for producing a photosynthetic culture according to one of claims 1 to 4, characterized in that said at least one light distributor (6) is provided with means (11, 11a, 11b, 11c, 11d ) fluid supply and extraction.
    [0006]
    6. Apparatus for producing a photosynthetic culture according to one of claims 1 to 5, characterized in that the fluid filling at least in part said sealed cavity is composed of water and air, able to pressurize the internal volume of said at least one light distributor (6) so as to seal the shape cooperation between the edge (18) of said opening (16) and said surface relief (17) of said at least one light distributor (6); ).
    [0007]
    7. A photosynthetic culture production device according to one of claims 1 to 6, characterized in that said at least one light distributor (6) is provided with an anchoring means (10) disposed at one end distal.
    [0008]
    8. Device for producing a photosynthetic culture according to one of claims 1 to 7, characterized in that said walls (7,8) of said at least one light distributor are formed by at least one flexible polymer membrane.
    [0009]
    9. Apparatus for producing a photosynthetic culture according to the preceding claim, characterized in that said flexible membrane has received or comprises during its manufacture at least one internal and / or external surface treatment among the following: anti-gout, anti dust, UV and / or infrared filter, UV stabilizers, diffusing effect, hydrophobic, anti-dust, anti-algae.
    [0010]
    10. Apparatus for producing a photosynthetic culture according to one of claims 1 to 9, characterized in that said photo-bioreactor (2) comprises at least one bubbling means (9) with CO2 input, disposed at least on part of the bottom of the enclosure (3) of the photo-bioreactor (2), so as to allow permanent mixing of said aqueous liquid comprising said photosynthetic culture.
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    同族专利:
    公开号 | 公开日
    CN107109321B|2020-11-13|
    CN107109321A|2017-08-29|
    BR112017011702A2|2018-02-27|
    US10287538B2|2019-05-14|
    WO2016087779A1|2016-06-09|
    US20170342361A1|2017-11-30|
    AU2015356934B2|2020-02-20|
    EP3227427A1|2017-10-11|
    EP3227427B1|2019-08-21|
    FR3029209B1|2018-02-09|
    AU2015356934A1|2017-06-22|
    ES2754265T3|2020-04-16|
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    法律状态:
    2015-11-03| PLFP| Fee payment|Year of fee payment: 2 |
    2016-06-03| PLSC| Publication of the preliminary search report|Effective date: 20160603 |
    2016-10-04| PLFP| Fee payment|Year of fee payment: 3 |
    2017-08-25| TP| Transmission of property|Owner name: SUNOLEO, FR Effective date: 20170726 |
    2017-09-15| PLFP| Fee payment|Year of fee payment: 4 |
    2018-09-21| PLFP| Fee payment|Year of fee payment: 5 |
    2019-09-27| PLFP| Fee payment|Year of fee payment: 6 |
    2021-09-10| ST| Notification of lapse|Effective date: 20210806 |
    优先权:
    申请号 | 申请日 | 专利标题
    FR1461778|2014-12-02|
    FR1461778A|FR3029209B1|2014-12-02|2014-12-02|DEVICE FOR PRODUCING PHOTOSYNTHETIC CULTURE USING A PHOTO-BIOREACTOR AND AT LEAST ONE LIGHT DISPENSER|FR1461778A| FR3029209B1|2014-12-02|2014-12-02|DEVICE FOR PRODUCING PHOTOSYNTHETIC CULTURE USING A PHOTO-BIOREACTOR AND AT LEAST ONE LIGHT DISPENSER|
    CN201580065541.5A| CN107109321B|2014-12-02|2015-12-02|Device for producing photosynthetic cultures by means of a photobioreactor and at least one light distributor|
    BR112017011702-9A| BR112017011702B1|2014-12-02|2015-12-02|DEVICE FOR PRODUCTION OF A PHOTOSYNTHETIC CULTURE|
    EP15810693.0A| EP3227427B1|2014-12-02|2015-12-02|Device for producing a photosynthetic culture by means of a photo-bioreactor and at least one light distributor|
    PCT/FR2015/053294| WO2016087779A1|2014-12-02|2015-12-02|Device for producing a photosynthetic culture by means of a photo-bioreactor and at least one light distributor|
    ES15810693T| ES2754265T3|2014-12-02|2015-12-02|Production device for a photosynthetic culture by means of a photobioreactor and at least one light distributor|
    AU2015356934A| AU2015356934B2|2014-12-02|2015-12-02|Device for producing a photosynthetic culture by means of a photo-bioreactor and at least one light distributor|
    US15/532,268| US10287538B2|2014-12-02|2015-12-02|Device for producing a photosynthetic culture by means of a photo-bioreactor and at least one light distributor|
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